国产无码

RUDN
RUDN
ENG
ENG
Research and Innovation
Laboratories and Centers
Single Cell Biology Laboratory
Single Cell Biology Laboratory
Medicine

Type

Laboratory

Department

Institute of Medicine

Head:

Evgeniy Denisov

Head of Laboratory

Contacts:

denisov-ev@rudn.ru

Structural unit: Institute of Medicine.

The Laboratory specializes in applying molecular genetic methods to study individual cells and bioinformatics and systems analysis approaches to understand the mechanisms of biological processes and the functioning of living organisms under both normal and pathological conditions.

Resources

Our laboratory facilities enable the use of the following research methods:

  • Single-cell DNA and RNA sequencing
  • Spatial transcriptomics
  • Whole-genome, whole-exome, whole-transcriptome, bisulfite and targeted sequencing
  • Bioinformatic analysis

Equipment Fleet

  • Chromium X Platform (10x Genomics, USA)
  • Genolab M Sequencing Platform (GeneMind Biosciences, China)
  • KF-PRO-005 Digital Pathology Scanner (KF-BIO, China)
  • Qsep400 Bio-Fragment Analyzer (BiOptic, China)
  • Fluo-100 Fluorometer (Allsheng, China)
  • DSC-400 Single Cell Suspension Dissociator (RWD Life Science, China)
  • GeneExplorer Thermal Cycler (Bioer, China)
  • Q2000 Real Time PCR (LongGene, China)

The team includes specialists in digital technologies, molecular biology and clinical medicine who have experience in big data analysis, molecular genetic and cellular research, and clinical patient management.

Capabilities

  • Detection of genetic variants (SNVs, indels, CNVs) and epigenetic modifications
  • Assessment of gene expression and non-coding RNAs
  • Detection and construction of gene regulatory networks
  • Identification of cell types and populations
  • Determination of intercellular (ligand-receptor) interactions
  • Analysis of cell differentiation (developmental) trajectories and phylogeny
  • Metagenome analysis

Partners

  • Tomsk National Research Medical Center of the Russian Academy of Sciences
  • Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology
  • N.N. Blokhin National Medical Research Center of Oncology
  • Ivannikov Institute for System Programming of the Russian Academy of Sciences
Main scientific directions
  • Development of聽a聽molecular atlas of聽malignant pediatric lymphoproliferative disorders
  • Creation of聽a聽molecular atlas of聽soft tissue sarcomas
Achievements
A聽unique methodology for single-cell DNA and RNA sequencing, spatial transcriptomics and bioinformatic analysis was developed to聽study the 鈥渕olecular portrait鈥� of聽each individual cell while preserving tissue structure and cell location.

It enables:

  • deciphering cellular heterogeneity,
  • understanding the organization of tissues and organs,
  • identifying the mechanisms of biological processes and cell origins,
  • identifying clinically significant cell populations, predictive and prognostic markers, and therapeutic targets.

This methodology is key to understanding the functioning of living organisms under normal and pathological conditions and developing fundamentally new approaches to personalized disease therapy.