RUDN University Biophysicist modelled the effect of antiseptics on bacterial membranes
Antiseptics are chemical agents that affect the internal processes or external structures of harmful microorganisms causing them to die. For example, alcohols break down important building and regulation blocks of bacteria and viruses. Other antiseptics target the integrity of bacterial membranes. They are effectively used against a wide range of pathogens, but their mode of action remains elusive. Scientists are aware of some general patterns, such as the presence of electrically charged particles in the molecules of antiseptic agents. The team developed a computer model of a bacterial membrane and found out the mechanism of the antiseptic activity. The results of the study can help to combat bacterial resistance.
“Some pathogens, especially those associated with hospital infections, show resistance to antiseptics. It is important to understand the physics behind the interaction of antiseptics and microorganisms to use antiseptics more efficiently and to develop new agents,” said professor Ilya Kovalenko, Ph.D., Doctor of Science in Physics and Mathematics, working under Project 5-100 at RUDN University.
The scientists developed a model of a bacterial membrane and put the molecules of four antiseptics (miramistin, chlorhexidine, picloxydine, and octenidine) on it. All these substances are cationic antiseptics, i.e. their molecules are positively charged. However, to the researchers’ surprise, the antiseptics failed to damage the membrane and just slightly changed its structure. Even when the ratio of antiseptics to membrane lipids was increased from 1/24 to 1/4, the membrane was not destroyed.
The destruction of the membrane took place only when an external electric field (with the intensity of 150 mV/nm) was added to the model. The membrane started to restructure, and pores began to form around the molecules of the antiseptics. Then, water got into them and made them bigger; and eventually, the membrane was torn apart. This was because the membrane became thinner around positively charged molecules: the molecules of the membrane had no charge and therefore were pushed away. An uneven membrane became more susceptible to adverse external factors, which led to the death of the cell.
“We studied the reaction of the model membrane to several cationic antiseptics and found out that structural changes in the membrane in the presence of an electrical field play a key role in the formation of pores. We plan to use this model to predict the effect of existing and new antiseptics on different microorganisms,” added professor Ilya Kovalenko, Ph.D., Doctor of Science in Physics and Mathematics, working under Project 5-100 at RUDN University.
The results of the study were published in .
The RUDN University Science and Innovation Prize winners were honoured at the extended meeting of the Academic Council. In 2024 the terms of the traditional RUDN University Prize were changed: for the first time the competition was announced in two categories: leading scientists and young scientists.
According to the International Energy Agency (IEA), electricity consumption in Africa has increased by more than 100% over the past two years (2020-2022). However, 74.9% of this energy is still produced by burning organic fuels 鈥� natural gas, coal and oil. At the same time, the level of electrification on the continent remains extremely low 鈥� only 24%, while in other developing countries it reaches 40%. Even in grid-connected areas, electricity supply is often unreliable: industrial enterprises lose energy on an average of 56 days a year.
Today, diagnosis and treatment planning with orthodontists takes several days. Also, complications can arise during treatment that slow down the patient's recovery process. For example, improper orthodontic treatment planning can lead to temporomandibular joint dysfunction.
The RUDN University Science and Innovation Prize winners were honoured at the extended meeting of the Academic Council. In 2024 the terms of the traditional RUDN University Prize were changed: for the first time the competition was announced in two categories: leading scientists and young scientists.
According to the International Energy Agency (IEA), electricity consumption in Africa has increased by more than 100% over the past two years (2020-2022). However, 74.9% of this energy is still produced by burning organic fuels 鈥� natural gas, coal and oil. At the same time, the level of electrification on the continent remains extremely low 鈥� only 24%, while in other developing countries it reaches 40%. Even in grid-connected areas, electricity supply is often unreliable: industrial enterprises lose energy on an average of 56 days a year.
Today, diagnosis and treatment planning with orthodontists takes several days. Also, complications can arise during treatment that slow down the patient's recovery process. For example, improper orthodontic treatment planning can lead to temporomandibular joint dysfunction.